Lencer
WI, Chu SH, Walker WA. 1987. Differential binding kinetics of cholera toxin
to developing intestinal microvillus membrane. Infection and Immunity 55: 3126-3130.
Verkman
A.S., Lencer W.I., Brown D., and D. Ausiello. 1988. Endosomes from kidney collecting
duct contain the vasopressin-sensitive water channel. Nature 333: 268- 269.
Ye
R., Shi L-b, Lencer W. I., and A. S. Verkman. 1989. Functional colocalization
of water channels and proton pumps in endosomes from kidney proximal tubule.
J. Gen. Physiol. 93: 885 - 902.
Lencer
W. I., F. Reinhart, and M. R. Neutra. 1990. Interaction of cholera toxin with
cloned human goblet cells in monolayer culture. Am. J. Physiol. (Gastrointest.
Liver Physiol). 258: G96 - G102.
Lencer
W. I., P. Weyer, A. S. Verkman, D. A. Ausiello, and D. Brown. 1990. FITC- dextran
as a probe for endosome function and localization in kidney. Am. J. Physiol.
(Cell Physiol.) 258: C309 - 317.
Shi
L-B., R-G. Ye, W. I. Lencer, and A. S. Verkman. 1990. Characterization of proton
pump and osmotic water transport in endocytic vesicles from rat kidney proximal
tubule. Acta Physiologica Sinica 42: 105-113.
Lencer,
W. I., A. S. Verkman, A. Arnaout, D. Ausiello, and D. Brown. 1990. Endocytic
Vesicles from Renal Papilla which Retrieve the Vasopressin-Sensitive Water Channel
do not contain an H+ATPase. J. Cell Biol. 111: 379-389.
Lencer,
W. I., D. Brown, D. Ausiello, and A. S. Verkman. 1990. Endocytosis of water
channels in rat kidney: cell specificity and correlation with in vivo antidiuresis.
Am. J. Physiol. (Cell Physiol.) 259: C920-C932.
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1991-1995
Madara,
J. L., C. Parkos, S. Colgan, R. J. MacLeod, S. Nash, J. Matthews, C. Delp, and
W. Lencer. 1992. Cl- secretion in a model intestinal epithelium induced by a
neutrophil derived secretagogue. J. Clin. Invest. 89: 1938-1944.
Lencer,
W. I., C. Delp, M. R. Neutra, J. L. Madara. 1992. Mechanism of cholera toxin
action on a polarized human intestinal cell line: role of vesicular transport.
J. Cell Biol. 117: 1197-1209.
Kendall,
D., Lencer, W., and K. Matlin. 1992. Cryosectioning of epithelial cells grown
on permeable supports. J. Tissue Culture Methods 14: 181-186.
Lencer,
W. I., J. B. de Almeida, S. Moe, J. L. Stow, D. A. Ausiello, and J. L. Madara.
1993. Entry of cholera toxin into polarized human intestinal epithelial cells:
Identification of an early brefeldin A sensitive event required for A1-peptide
generation. J. Clin. Invest. 92: 2941-2951.
Apter, F. M., W. I. Lencer, R. A. Finkelstein, J. J. Mekalanos and M. R. Neutra.
1993. Monoclonal antibodies directed against cholera toxin prevent the toxin-induced
secretory response and block toxin binding to intestinal epithelial cells in
vitro. Infect. Immun. 61: 5271-5278.
Dosanjh, A., W. I. Lencer, D. Brown, D. A. Ausiello, J. L. Stow. 1994. Heterologous
expression of ?F508 CFTR results in decreased sialylation of membrane glycoconjugates.
Am. J. Physiol (Cell). 266: C360-C366.
Strohmeier, G. R., Reppert, S., Lencer, W. I., and J. L. Madara. 1995. The A2b
adenosine receptor mediates cAMP responses to adenosine receptor agonists in
human intestinal epithelia. J. Biol. Chem. 270: 2387-2394.
Lencer, W. I., G. Strohmeier, S. Moe, S. L. Carlson, C. T. Constable, and J.
L. Madara. 1995. Signal transduction by cholera toxin in polarized epithelia:
processing in vesicular compartments does not require acidification. Am. J.
Physiol. 269: G548-G557.
Lencer, W. I., S. Moe, P. A. Rufo, and J. L. Madara. 1995. Transcytosis of cholera
toxin subunits across model human intestinal epithelia. Proc. Natl. Acad. Sci.
USA. 92: 10094-10098.
Lencer, W. I., C. Constable, S. Moe, M. Jobling, H. M. Webb, S. Ruston, J. L.
Madara, T. Hirst, and R. Holmes. 1995. Targeting of cholera toxin and E. coli
heat labile toxin in polarized epithelia: role of C-terminal KDEL. J. Cell Biol.
131: 951-962.
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1996-2000
Turner,
J.R., W. I. Lencer, S. Carlson, and J. L. Madara. 1996. Carboxy terminus VSV-G
tagged intestinal Na+-glucose cotransporter (SGLT1): Maintenance of surface
expression and global transport function with selective perturbation of transport
kinetics and polarized expression. J. Biol. Chem. 271: 7738-7744.
Frey,
A., K. T. Giannasca, R. Weltzin, P. J. Giannasca, H. Reggio, W. I. Lencer, and
M. R. Neutra. 1996. Role of the glycocalyx in regulating access of microparticles
to apical plasma membranes on intestinal epithelial cells: implications for
microbial attachment and oral vaccine targeting. J. Exp. Med. 184: 1045 - 1059.
Rufo, P. A., L. Jang, S. J. Moe, C. Brungara, S. L. Alper, and W. I. Lencer.
1996. The antifungal antibiotic clotrimazole inhibits Cl- secretion by polarized
monolayers of human colonic epithelial cells. J. Clin. Invest. 98: 2066 - 2075.
Strohmeier, G. R., W. I. Lencer, T. W. Patapoff, L. F. Thompson, S. L. Carlson,
S. J. Moe, D. Carnes, R. J. Mrsny, and J. L. Madara. 1997. Surface expression,
polarization, and functional significance of CD73 in human intestinal epithelia.
J. Clin. Invest. 99: 2588-2601.
Lencer, W. I., G. Cheung, G. R. Strohmeier, M. Currie, A. J. Ouellette, M. E.
Selsted, and J. L. Madara. 1997. Induction of epithelial Cl secretion by channel
forming cryptdins 2 and 3. Proc. Natl. Acad. Sci. USA. 94: 8585-8589. (Anonymous
Submission)
Lencer, W. I., C. Constable, S. Moe, P. A. Rufo, A. Wolf, M. G. Jobling, S.
P. Ruston, J. L. Madara, R. K. Holmes, and T. R. Hirst. 1997. Proteolytic activation
of cholera toxin and Escherichia coli labile toxin by entry into host epithelial
cells: signal transduction by a protease-resistant toxin variant. J. Biol. Chem.
272: 15562-15568.
Rufo, P. A., D. Merlin, M. Riegler, M. Ferguson-Maltzman, B. Dickinson, C. Brugnara,
S. L. Alper, and W. I. Lencer. 1997. The antifungal antibiotic clotrimazole
inhibits chloride secretion by human T84 cells via blockade of distinct basolateral
K+ conductances. J. Clin. Invest. 100: 3111-3120.
Wolf, A. A., M. G. Jobling, S. Wimer-Mackin, J. L. Madara, R. K. Holmes, and
W. I. Lencer. 1998. Ganglioside structure dictates signal transduction by cholera
toxin in polarized epithelia and association with caveolae-like membrane domains.
J. Cell Biol. 141:917-927.
Rodighiero, C., A.T. Aman, M.J. Kenny, J. Moss, W.I. Lencer, and T.R. Hirst.
1999. Structural basis for the differential toxicity of cholera toxin and E.
coli heat-labile enterotoxin. J. Biol. Chem. 274:3962-3969.
Beauregard, K., S. Wimer-Mackin, R.J. Collier, and W.I. Lencer. 1999. Entry
of anthrax toxin into a polarized human epithelial cell line: strict dependence
on basolateral membrane receptors. Infect. Immun. 67: 3026-3030.
Merlin, D., L. Jiang, G.R. Strohmeier, A. Nusrat, S.L. Alper, W.I. Lencer, and
J.L. Madara. 1998. Distinct Ca2+- and cAMP-dependent anion conductances in the
apical membrane of polarized T84 cells. Am. J. Physiol. (Cell Physiol.). 275:C484-C495.
Dickinson, B., K. Badizadegan, Z. Wu, J.C. Ahouse, X. Zhu, N.E. Simister, R.S.
Blumberg, and W.I. Lencer. 1999. Bidirectional FcRn-dependent IgG transport
in a polarized human intestinal cell line. J. Clin. Invest. 104: 903-911.
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2000-Present
Badizadegan, K., B.L. Dickinson, H.E. Wheeler, R.S. Blumberg, R. K. Holmes,
and W.I. Lencer. 2000. Hetergeneity of detergent insoluble membranes from human
epithelia containing caveolin-1 and ganglioside GM1. Am. J. Physiol. 278: G895-G904.
Mel, S.F., K.J. Fullner, S. Wimer-Mackin, W.I. Lencer, and J.J. Mekalanos. 2000.
Association of Protease Activity in Vibrio cholerae Vaccine Strains with Decreases
in Transcellular Epithelial Resistance of Polarized T84 Intestinal Epithelial
Cells. Infect. Immun. 68: 6487-92.
Merlin, D., W.I. Lencer, M.E. Selsted, and J.L. Madara. 2000. Cryptdin-3 induces
novel apical anion conductances in CT secretory epithelia. Am. J. Physiology
(Cell). 280: C296-302
Tsai, B., C. Rodighiero, W.I. Lencer, and T. Rapoport. 2001. Protein disulfide
isomerase acts as a redox-dependent chaperone to unfold cholera toxin. Cell.
104:937-948.
Wimer-Mackin, S., R.K. Holmes, A.A. Wolf, W.I. Lencer, and M.G. Jobling. 2001.
Characterization of receptor-mediated signal transduction by Escherichia coli
type IIa heat-labile enterotoxin in the polarized human intestinal cell line
T84. Infect Immun. 69:7205-12.
Zhu,
X., G. Meng, B.L. Dickinson, X. Li, E. Mizoguchi, L. Miao, Y. Wang, C. Robert,
B. Wu, P.D. Smith, W.I. Lencer, and R.S. Blumberg. 2001. MHC class I-related
neonatal Fc receptor for IgG is functionally expressed in monocytes, intestinal
macrophages, and dendritic cells. J Immunol. 166:3266-76.
Merlin,
D., W.I. Lencer, M.E. Selsted, and J.L. Madara. 2001. Cryptdin-3 induces novel
apical anion conductances in CT secretory epithelia. Am. J. Physiology (Cell).
280:C296-302.
Aman, A. T., S. Fraser, E.A. Merritt, C. Rodigherio, M.J. Kenny, M. Ahn, W.G.
Hol, N.A. Williams, W.I. Lencer, and T.R. Hirst. 2001. A mutant cholera toxin
B-subunit that binds GM1-ganglioside but lacks immunomodulatory or toxic activity.
Proc Natl Acad Sci U S A. 98: 8536-41.
Fullner, K.J., W.I. Lencer, and J.J. Mekalanos. 2001. Vibrio cholerae-induced
cellular responses of polarized T84 intestinal epithelial cells are dependent
on production of cholera toxin and the RTX toxin. Infect Immun. 69:6310-7.
Rodighiero,
C., Y. Fujinaga, T.R. Hirst, and W.I. Lencer. 2001. A cholera toxin B-subunit
variant that binds ganglioside GM1 but fails to induce toxicity. J Biol Chem.
276:36939-45.
Wolf, A. A. Y. Fujinaga, and W I. Lencer. 2002. Uncoupling of the cholera toxin
GM1 ganglioside-receptor complex from endocytosis, retrograde Golgi trafficking,
and downstream signal transduction by depletion of membrane cholesterol. J.
Biol. Chem. 277:16249-56.
Yue,
G., D. Merlin, M.E. Selsted, W.I. Lencer, J.L. Madara, and D.C. Eaton. 2002.
Cryptdin 3 forms anion selective channels in cytoplasmic membranes of human
embryonic kidney cells. Am J Physiol Gastrointest Liver Physiol. 282:G757-65.
Claypool, S.M., Dickinson, B.L., Yoshida, M., Lencer, W.I., and Blumberg, R.S.
2002. Functional reconstitution of human FcRn in Madin-Darby canine kidney cells
requires co-expressed human beta 2-microglobulin. J Biol Chem 277:28038-28050.
Spiekermann, G.M., Finn, P.W., Ward, E.S., Dumont, J., Dickinson, B.L., Blumberg,
R.S., and Lencer, W.I. 2002. Receptor-mediated immunoglobulin G transport across
mucosal barriers in adult life: functional expression of FcRn in the mammalian
lung. J Exp Med 196:303-310.
Zhu, X., J. Peng, R. Raychowdhury, A. Nakajima, W.I. Lencer, and R.S. Blumberg.
2002. The heavy chain of neonatal Fc receptor for IgG is sequestered in endoplasmic
reticulum by forming oligomers in the absence of beta2-microglobulin association.
Biochem J. 367:703-14.
Rodighiero, C., Tsai, B., Rapoport, T. A., and Lencer, W. I. 2002. Role of ubiquitination
in retro-translocation of cholera toxin and escape of cytosolic degradation.
EMBO Reports 3, 1222-1227.
Shah, U., B.L. Dickinson, R.S. Blumberg, N.E. Simister, W.I. Lencer, and W.A.
Walker. 2003. Distribution of the IgG Fc receptor, FcRn, in the human fetal
intestine. Pediatr Res. 53:295-301.
Fujinaga, Y., A.A. Wolf, C. Rodigherio, H. Wheeler, B. Tsai, L. Allen, M.G.
Jobling, T. Rapoport, R.K. Holmes, and W.I. Lencer. 2003. Gangliosides that
associate with lipid rafts mediate transport of cholera toxin from the plasma
membrane to the ER. Mol Biol Cell. 14: 4783-93
Tsai, B., J.M. Gilbert, T. Stehle, W. Lencer, T.L. Benjamin, and T.A. Rapoport.
2003. Gangliosides are receptors for murine polyoma virus and SV40. Embo J.
22:4346-4355.
Rufo, P.A., P.W. Lin, A. Andrade, L. Jiang, L. Rameh, C. Flexner, S.L. Alper,
and W.I. Lencer. 2003. Diarrhea-associated HIV-1 aspartyl protease-inhibitors
potentiate muscarinic activation of Cl- secretion by T84 cells via prolongation
of cytosolic Ca2+ signaling. Am. J. Physiol. Cell. in press.
Claypool, S., B. Dickinson, J. Wagoner, W.I. Lencer, and R.S. Blumberg. 2003.
Bidirectional transport of IgG by a strongly polarized basolateral Fc-receptor.
Mol. Biol. Cell in press.
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Selected
Reviews, Editorials, and Book Chapters
Lencer,
W.I., T.R. Hirst, and R.K. Holmes. 1999. Membrane traffic and the cellular uptake
of cholera toxin. Biochim. Biophys. Acta. (Molecular Cell Research). 1450: 177-190.
(invited review)
Blumberg, R.S., W.I. Lencer, X. Zhu, H.-S. Kim, S. Claypool, S.P. Balk, L.J.
Saubermann, and S.P. Colgan. 1999. Antigen presentation by intestinal epithelial
cells. Immunology Letters. 69:7-11.
Lencer,
W.I. 2000. Microbial invasion across the epithelial barrier. Pediatric Research.
49:4-5.
Lencer, W.I. 2001. Cholera: Invasion of the intestinal epithelial barrier by
a stably folded protein toxin. Am J Physiol Gastrointest Liver Physiol. 280:G781-6.
(invited review)
Badizadegan,
K., Collier, R.J., and Lencer, W.I. 2002. Membrane translocation by bacterial
toxins. In Methods in Microbiology. P. Sansonetti, and A. Zychlinsky, editors.
San Diego: Academic Press. 277-296.
Bonny
L. Dickinson and Wayne I. Lencer. 2003. Transcytosis of Bacterial Toxins Across
Mucosal Barriers in Bacterial Protein Toxins D, Burns, J. Barbieri, B. H. Iglewski,
and R. Rappuoli editors. ASM Press. Washington D.C. .
Chiara Rodighiero and Wayne I. Lencer. 2003. "Trafficking of cholera and
related bacterial enterotoxins: pathways and endpoints" in Microbial Pathogens
and the Intestinal Epithelial Cell. Gail Hecht, editor, ASM Press.
Lencer, W.I., K. Barrett, S. Colgan, M.B. Cohen, C.P. Duggan, M. Kotelchuck,
and J.M. Rhoads. 2002. Research Agenda for Pediatric Gastroenterology, Hepatology
and Nutrition: Secretion and Diarrhea: Report of the North American Society
for Pediatric Gastroenterology, Hepatology and Nutrition for the Children's
Digestive Health and Nutrition Foundation. J Pediatr Gastroenterol Nutr. 35
Suppl:S246-9.
Lencer, W.I., and B. Tsai. 2003. The intracellular voyage of cholera toxin:
Going retro. Trends Biol Chem. 28: 639-645. (invited review)
