Name: Wayne Isaac Lencer
Office Address: GI Cell Biology - Enders 720
Division of Gastroenterology and Nutrition
Children's Hospital
300 Longwood Ave.
Boston , MA 02115
617-919-2573
wayne.lencer@childrens.harvard.edu
Home Address:
60 Louders Lane
Jamaica Plain , MA 02130
Date of Birth: September 22, 1950
Birth Place: Miami , Florida
Education:
1972 B.A. University of Michigan
1977 M.D. Boston University School of Medicine Medicine
Postdoctoral Training:
Internship and Residency:
1977-1980 PL1, PL2, PL3 Children's Service, Boston City Hospital
Research and Fellowship:
1981 Clinical Fellow, Newborn Medicine, Boston Perinatal Center, Boston City Hospital
1984-87 Clinical and Research Fellow, Combined Program in Pediatric Gastroenterology and Nutrition, The Children's Hospital and Massachusetts General Hospital , Boston
1987-1990 Post-Doctoral fellowship in Biophysics and Cell Biology, Drs. Ausiello and Brown, Massachusetts General Hospital, Harvard Medical School, and Dr. A. S. Verkman, Univ. of California, San Francisco
Licensure and Certification:
1978 Massachusetts Medical Liscense No. 45839
1978 Diplomate, National Board of Medical Examiners No. 172210
1983 American Academy of Pediatrics, Board Certified in Pediatrics. No. 028570
1991 American Academy of Pediatrics, Board Certified in Gastroenterology No. 44359
• American Academy of Pediatrics , Re-certified in Gastroenterology No. 44359
2002 American Board of Pediatrics, Re-certified in Gastroenterology No.44359
Academic Appointments:
1982-84 Clinical Instructor, Children's Service
Boston University School of Medicine, Boston , MA
1984-87 Clinical and Research Fellow in Pediatrics
Harvard Medical School , Boston , MA
1987-91 Instructor in Pediatrics
Harvard Medical School , Boston , MA
1991-97 Assistant Professor in Pediatrics
Harvard Medical School , Boston , MA
1997- Associate Professor in Pediatrics
Harvard Medical School , Boston , MA
Hospital or Affiliated Institution Appointments:
1982-84 Assistant Visiting Physician, Pediatrics Boston City Hospital , Boston , MA
1984-87 Fellow in Pediatrics, Massachusetts General Hospital, The Children's Hospital, and Shriners Burns Institute, Boston
1987- Assistant Pediatrician, Massachusetts General Hospital , Boston , MA
1987-96 Assistant in Medicine, The Children's Hospital, Boston , MA
1988-89 Assistant Pediatrician, Rhode Island Hospital , Providence RI
1990- Research Associate, Pathology, Brigham and Women's Hospital
1996- Associate in Medicine, The Children's Hospital, Boston , MA
2004 Chief, Division of Gastroenterology and Nutrition, Children's Hospital Boston
Other Professional Appointments and Major Visiting Appointments
1980 Staff Pediatrician, Khao I Dang Field Hospital, United Nations High Commission for Refugees, Prachinburi Province , Thailand
1982-84 Medical Director and Senior Pediatrician, Whittier Street Neighborhood Health Center , Roxbury , MA
Hospital and Health Care Organization Service Responsibilities
1988-89 Pediatric Gastroenterologist Rhode Island Hospital
1989 - Pediatric Gastroenterologist, Attending Outpatient Clinic, Children's Hospital, Boston
1986 - Pediatric Gastroenterologist, Attending Nutrition Support Service, Children's Hospital, Boston
Major Administrative Responsibilities
1997- Associate Director, Harvard Digestive Diseases Center
Director Core C: Epithelial Cell Function
NIH Center Grant
• Director, Harvard Medical School Fellowship in Pediatric Gastroenterology and Nutrition
2004 Chief, Division of Gastroenterology and Nutrition, Children's Hospital, Boston
Major Committee Assignments
Medical/Dental school
1996- Harvard Digestive Diseases Center : Enrichment program, Chair
1997- Co-Director, Harvard Digestive Diseases Center
1997- Director Core C in Epithelial Cell Function, NIH Center Grant
Affiliated Institutions
1994- Children's Hospital GI Fellowship Training Program: Faculty, academic enrichment program.
2000 - 01 Committee on Faculty Education, Dept. of Medicine, Childrens Hospital
2001- Research Operations Committee, Enders, Children's Hospital
Regional
2002 Ad Hoc Hood Foundation Study Section Member
2004 Scientific Advisory Board, Syntonix Pharmaceuticals Inc. Waltham MA
2004 - Scientific Advisory Board, NIH GRASP Center , NEMC
National
1996 NIH Study Section Ad Hoc member- Bacteriology & Mycology I
1999 Chair, Subcommittee on Secretion and Diarrheal Diseases of the Task Force on Research Agenda for The Children's Digestive Health and Nutrition Foundation
2000 NIH Study Section RFA DK-00-005 "Foodborne Illnesses, Gastrointestinal and Renal Complications"
2001 NIH Study Section Member PA- AI-01-075 "Integrated Preclinical/clinical program for HIV topical microbicides"
2001 Research Committee and Study Section Member of NASPGHAN the North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition
2001 NIH Study Section Permanent Member NIDDK-C
2003 Study Section Permanent Member: Crohn's and Colitis Foundation Senior Research Awards
Professional Societies
1983- Fellow, American Academy of Pediatrics
1987- Member, American Gastroenterology Association
1990 - Member, Gastroenterology Research Group
1990 - Member, American Society for Cell Biology
1990 - Member, American Association for the Advancement of Science
1993 - Elected Member, Salt and Water Club
1993 - Elected Member, Society for Pediatric Research
1996 - Member, North American Society for Pediatric Gastroenterology and Nutrition
2002- American Society for Biochemistry and Molecular Biology (ASBMB). Nominated by Dr. Martha Vaughn
2004 - Elected Fellow, American Association for the Advancement of Science
Community Service Related to Professional Work:
Editorial Boards:
2000 Editorial Board, American Journal of Physiology: Gastrointestinal and Liver Physiology
Awards and Honors:
1968-72 Class Honors all years, University of Michigan
1970 James B. Angell Scholar, University of Michigan
1972 Phi Beta Kappa, B.A. with High Distinction, University of Michigan
1977 Pediatric Departmental Award for "Outstanding performance in all aspects of Pediatric undergraduate education", Boston University School of Medicine
1988 Charles Hood Foundation Career Development Award
1989 Clinical Investigator Award: NIH, Bethesda ML
1996 Miles and Shirley Fitterman/American Digestive Health Foundation: Samuel J. Meltzer Award for Basic Research in Gastroenterology
2003 2003 Nominee, Harvard Medical School Prize for Excellence in Teaching (HMS years 1 & 2)
2003 Excellence in Teaching Award, First Year Class HMS
2004 Elected Fellow of AAAS, American Association for the Advancement of Science
Narrative report 
My laboratory studies the cell and molecular biology of vesicular transport in polarized epithelial cells and regulation of ion transport in the intestine. These projects relate to how intestinal epithelial cells interact with the luminal and sub-epithelial microenvironment, and to the biology of bacterial pathogenesis and mucosal host defense. We have discovered how some enteric bacterial toxins breech the intestinal epithelial barrier and enter host epithelial cells to cause disease. These toxins hijack the cellular and molecular mechanisms of retrograde membrane transport to move from the luminal cell surface into the endoplasmic reticulum (ER) of affected cells, the total reverse of protein biosynthesis. In another project, the lab studies the cell and molecular biology of the MHC Class I-like IgG receptor FcRn. FcRn transports IgG across mucosal surfaces where it may function in immune surveillance and host defense. In a third area of interest, the lab aims to understand the mechanisms and regulation of intestinal Cl - secretion, the initial ion transport event in secretory diarrhea.
B. Funding Information
Previous Research Funding
1988-89 Career Development Award, Charles H. Hood Foundation
“Transepithelial Targeting of Cholera Toxin”
P.I. W. I. Lencer
1989-93 NIH KO8 Clinical Investigator Award, NIH DK01848
“Transepithelial Targeting of Cholera Toxin”
P.I.: W. I. Lencer
1991-93 Pilot Feasibility Study, Harvard Digestive Diseases Center
“Development of semipermeable intestinal cell systems using streptolysin-O toxin”
P.I.: W. I. Lencer (competitively renewed for second year)
1993-96 NIH Grant RO1 DK48106 - 01 - 03
"Intestinal Disease: Enterocyte/Toxin Interaction"
P.I.: W. I. Lencer
1994-98 NIH Grant Project #2
"Cryptdin effects on crypt epithelium"
P.I.: J. L. Madara (50%)
Co-P.I.: W. I. Lencer (50%)
1993 Milton Fund, Harvard Mediacl School
"Intestinal Disease: Enterocyte/Toxin Interaction"
P.I.: W. I. Lencer
1995 Pilot Feasibility Study, Ender's Faculty Research Council Award, Children's Hospital.
“Detergent-resistant membrane domains (caveolae): structure/function in polarized human intestinal epithelia”
P.I.: W. I. Lencer
1996 Career Development Award for Excellence in Basic Research: Miles and Shirley Fiterman Foundation/American Digestive Health Foundation/American Gastroenterology Association.
“Detergent-resistant membrane domains (caveolae): structure/function in polarized human intestinal epithelia”
P.I.: W. I. Lencer
1996-97 Sponsored Research Program-- Ion Technologies, Cambridge , MA : “Intestinal Cl secretion: Inhibitionby imidazole antibiotics” P.I.: W. I. Lencer
1996-97 Pilot Feasibility Study, Harvard Digestive Diseases Center
“Receptor-mediated transcytosis of IgG in adult life”
shared P.I.'s: R. S. Blumberg and W. I. Lencer
1998-‘99 NIH Grant P30 DK34854 Harvard Digestive Diseases Center
"Biology of Alimentary Tract in Heath and Disease"
P.I.: M. R. Neutra (Director)
W. I. Lencer (Co-Director)
Current Research Funding
1993-06 NIH Grant RO1 DK48106 – 12S – 13S supplement
"Genetic Analysis for Cell Invasion by Bacterial Toxins"
P.I.: W. I. Lencer
1993-06 NIH Grant RO1 DK48106 - 08 - 13
"Intestinal Disease: Enterocyte/Toxin Interaction"
P.I.: W. I. Lencer
1997-06 NIH Grant RO1 DK53056
"Intestinal Transcytosis of IgG in Adult Life"
P.I.: R. S. Blumberg (50%)
Co-P.I. W. I. Lencer (50%)
999-09 NIH Grant P30 DK34854 Harvard Digestive Diseases Center
"Biology of Alimentary Tract in Heath and Disease"
P.I.: M. R. Neutra (Director)
W. I. Lencer (Associate Director)
2000-05 NIH Grant RO1 DK57827
"Cryptdin effects on crypt epithelium"
P.I.: W. I. Lencer
2000-004 Sponsored Research Program—ICAgen Pharmaceuticals, Res. Triangle, NC “Inhibition of Intestinal Cl secretion by imidazole antibiotics”
P.I.: W. I. Lencer
• Sponsored Research Program—Pfizer, Treatment of HIV-1 Aspartyl-protease inhibitor induced secretory diarrhea
P.I.: W. I. Lencer
2000-09 NIH Grant T32 DK007477
Training in Pediatric Gastroenterology and Nutrition
P.I.: W. I. Lencer
Report of Teaching :
Local Contributions:
Teaching at Harvard Medical School and Children's Hospital
1982 - 84 Primary Care Preceptor, Harvard Medical School
One medical student precepted for 2 - 3 sessions in an outpatient setting (Whittier St. Health Center). Two students per year.
1993-current HST 120, Lecturer, Harvard-MIT program in Health Sciences Technology, Harvard Medical School and Massachusetts Institute of Technology, Gastrointestinal Physiology
Dr. Martin Carey, Professor in Medicine, HMS, Course Director
“Anatomy, physiology, and biophysical chemistry of terminal digestion, Parts I & II.
1993-94 Lecturer, Human Systems, Harvard Medical School , Dr. Julian Seifter, course director.
-Lecture entitled “Host-pathogen interactions in the gut” One lecture.
1995-current Series Organizer: Clinical/Basic Science Research Seminars
Division of Gastroenterology and Nutrition, Childrens Hospital .
1998-99 Human Systems, GI section, Harvard Medical School
Tutor, Course Director: Helen Shields
1998-current Harvard Medical School Integrated Human Physiology, HMS Year 1
Section Director for GI Physiology, 4 lectures and entire curriculum
Course Director: Richard Schwartzstein
2001-current Teaching Attending, Residency Program General Medicine, Childrens Hospital
2003-current Harvard Medical School BBS Microbiology
Bacterial Toxins, 1 lecture
Course Director: Steve Lory, PhD
Clinical Advisory and Supervisory Responsibilities
1982 - 84 Clinical Attending, Residency Training Program; Boston City Hospital, One month of ward attending, 6 residents, one month per year
1988 - 90 Staff Attending: Post-doctoral Training in Gastroenterology and Nutrition; Rhode Island Hospital , Brown Medical School
Intermittent clinical precepting of residents during GI consultations on ward service
1987- Staff Attending, Post-doctoral Training in Gastroenterology and /Nutrition, Children's Hospital and Massachusetts General Hospital , Harvard Medical School .
Laboratory and Advisory and Supervisory Responsibilities
1993- 1995 Principal Investigator, GI Cell Biology, Brigham and Women's Hospital
• Principal Investigator, GI Cell Biology, Children's Hospital
2002 Director, Harvard Medical School Fellowship in Pediatric Gastroenterology and Nutrition (NIH, T32 training grant)
Names of Advisees or Trainees:
1993- 1996 Signa Moe (research associate).
Current Position : M.D./Ph.D. Resident in Internal Medicine, Univ. of Seattle Medical School , WA .
1994- Paul Rufo, M.D . (Post-doctoral fellow).
Current Position : Instructor in Pediatrics at Harvard Medical School
Awards and Funding History : AGA Foundation grant; Galaxo Research Training Award; NIN K23 Career Development Award; Pfizer Research Grant; FDA Orphan Drug Grant;
1995-1996 Gilbert Cheung (MIT undergraduate student).
Current Position : MD Washington University , St. Louis .
1996- Anne Wolf, M.D . (Post-doctoral fellow).
Current Position : Instructor in Pediatrics, Harvard Medical School
Awards and Funding History : NIH Individaul NRSA; ADHF Research Training Award; ADHF Young Investigator Award; NIH K08 Career Development Award; NASPGHAN Research Fellow Award; NASPGHAN Young Investigator Award;
• Susan Wimer-Mackin, Ph.D . (Post-doctoral fellow).
Current Position : Program Director, Senior Scientist LigoCyte Pharmaceuticals, Bozeman , Montana
• Kate Beauregard , Secondary Mentor and Doctoral thesis committee in Biological and Biomedical Sciences, Harvard Medical School .
1997-2004 Bonny Dickinson, Ph.D . (Post-doctoral fellow).
Current Position : Assistant Professor, Microbiology, Univ. of Lousiana Medical School, New Orleans
Awards and Funding History : NIH Individual NRSA; NIH K01 Career Development Award; Crohns Colitis Foundation Sr. Investigator Award.
1997-2002 Kamran Badizadegan, M.D . (Post-doctoral fellow).
Current Position : Assistant professor in Pathology, Harvard Medical School and HST program MIT.
Awards and Funding History : NIH K08 Career Development Award
1997-1999 Larry Allen , (Medical Student HMS)
Current Position : Senior Resident MGH; PHS Masters degree in Medicine; Cardiology Fellow, Duke Univ.
1999 2002 Patty Lin , M.D. (Post-Doctoral Fellow in Neonatology)
Current Position ; Assistant professor in Pediatrics, Emory University
Awards and Funding History : NIH K08 Career Development Award; Nestle Research Fellow Award ; SSPR 2004 Young Faculty Award
1999 2002 Yukako Fujinaga , Ph.D.
Current Position , Assistant Professor in Microbiology, Osaka University , Japan
Awards and Funding History : Senior Independent Research Award, Ministry of Health , Japan
1999 2002 Chiara Rodighiero , Ph.D. (Post-doctoral Fellow in Cell Biology).
Current Position , Research Scientist and Project Director, DNA systems, Oxford , England
1999-2001 Gerburg Spiekermann , MD (Post-doctoral Fellow)
Current position : Assistant Professor, Research Associate, Department of Nutrition, Cornell University
Awards and Funding History : ADHF Research Training Award
• 2002 Peter Simon (undergraduate student) Current position: Univ .Virginia Medical School
2001-2004 William Ciesla , MD (Post-doctoral fellow).
Awards and Funding History : NIH K08 Career Development Award;
1998-2003 Steve Claypool , Collaborating Mentor for doctoral thesis in Immunology, Harvard Medical School .
Current position . Post doctoral fellow UCLA, Mentor Dr. Carla Koehler, Ph.D.
2002-04 Henry Pelish Collaborating Mentor for doctoral thesis in Chemistry
Current position . Post doctoral fellow Cell Biology HMS, Mentor Dr. Tomas Kirchhausen, Ph.D.
• David Saslowsky PhD. Post-doctoral fellow
2003 2004 Michael Kothe , PhD. Post-doctoral Fellow.
Current position . Research Scientist Structural Biology, Pfizer, Cambridge MA
2003 2004 Krishna Reddy , HMS Medical Student
2004 Elizabeth Yen , MD Post-doctoral Fellow
Regional, National, or International Contributions:
Regional
1989 Research Seminar, Dept. of Pediatrics , Massachusetts General Hospital
1990 Research Seminar, Harvard Digestive Diseases Center , Harvard Medical School
1990 Research Seminar, Renal Unit and Dept of Molecular Medicine, Beth Israel Hospital
1990 Research Seminar, Dept. of Pathology, Brighams and Womens Hospital
1992 Research Seminar, Dept. of Pathology, Brigham and Women's Hospital
1993 Research Seminar, Dept. of Surgery, Shrine Burns Hospital , MGH
1993 Research Seminar, Harvard Digestive Disease Center , Harvard Medical School
1996 Research Seminar, Membrane Biology Seminar Series, MIT/Harvard Medical School on “Cell biology of microbial toxins”.
1997 Research Seminar, Harvard Digestive Diseases Center , Harvard Medical School
1997 Research Seminar, Immunology Research Group, Cox Therhorst, Chair, Harvard Medical School
1997 Research Seminar, Gastroenterology Research Group, Tom LaMont, Chair, Beth Israel Hospital , Harvard Medical School
1997 Platform Presentation: Salt and Water Club Fall Meeting, MGH
1999 Harvard Institute of Medicine, Membrane Biology Series. Signal transduction by cholera toxin in polarized cells: association with caveolae-like membrane domains.
2000 Invited Speaker: Microbiology Department, Tuft's New England Medical Center
2000 Invited Speaker: Surgical Research Seminars, Beth Israel Deaconess Medical Center
2001 Invited Research Seminar: GI Research Unit, MGH
2001 Invited Research Seminar: Membrane Biology Program, Harvard medical School
2002 Grand Rounds, Childrens Hospital , Boston , Harvard Med. Sch.
2002 Grand Rounds, MGH Dept. of Pediatrics, Harvard Med. Sch.
2003 Invited seminar: Lipid Biochemistry, Boston wide symposium series by MIT, BU, Harvard, Tufts, Brandeis and U. Mass Medical School
2003 Grand Rounds, Boston Medical Center , Dept. of Pediatrics
2003 Research Seminar, GI Division, MGH Dept. of Medicine, Harvard Med. Sch.
2003 Research Seminar, GI Division, BWH Dept. of Medicine,
2004 Invited Research Seminar: Penobscot Bay Medical center
2004 Invited Research Seminar: Broad Research Institute, MIT, Boston
2004 Invited Research Seminar: BBS Graduate Program, HMS, Boston
2005 Research Seminar, GI Program Beth Israel Hospital
2005 Grand Rounds Research Seminar, Childrens Hospital Boston
National
1987 Pediatric Grand Rounds; University of Texas , Galveston
1988 Platform Presentation: Salt and Water Club, Dartmouth College
1989 Pediatric Grand Rounds
-Dept. of Pediatrics, Univ. of California , San Francisco
- Dept. of Pediatrics , Rhode Island Hospital, Brown University Sch. of Medicine
1989 Research Seminar, Renal Unit, Dept. of Medicine and GI/Nutrition, Dept. of Pediatrics, Univ. of California , San Francisco
1989 Platform Presentation: American Assoc. of Nephrology, Washington D.C.
“Water channels in collecting duct and toad bladder are present in a non- acidifying endosomal compartment”
1992 Research Seminar, Dept. of Pediatrics, Children's Hospital, Washington University Medical School , St. Louis
1993 Platform Presentation: American Gastroenterology Association, Spring Meetings. “Entry of cholera toxin into intestinal epithelial cells: role of vesicular traffic”
1993 Platform Presentation: Joint session of American Society for Clinical Investigation and Society for Pediatric Research, Spring Meetings. “Entry of cholera toxin into intestinal epithelial cells”
1993 Invited Speaker, National Scientific Symposium entitled Intestinal Transport: New Science. Organizers: M. Field and E. B. Chang. Sponsored by the Gastroenterology Research Group of the American Gastroenterological Association.
1994 Platform Presentation: American Gastroenterology Association Spring Meetings (two presentations). “Transcytosis of cholera toxin across the human intestinal epithelial cell line T84” and “Signal transduction by cholera toxin does not depend on toxin entry into acidic compartments”
1994 Invited Speaker, National Congress on Cell and Tissue Culture. Symposium entitled “In vitro assessment of transport mechanisms in mammalian cells”
1994 Invited Speaker, Fall Meetings of American Society for Microbiology, ACLAC. Symposium entitled “Cell biology of Microbial Toxins”
1995 Platform Presentation: American Gastroenterology Association Spring Meetings. “Targeting of cholera toxin and E. coli labile toxin in polarized epithelia: role of C-terminal KDEL”.
1996 Platform Presentation: American Gastroenterology Association Spring Meetings. “Proteolytic activation of cholera toxin and E. coli labile toxin by intestinal epithelia”.
1996 Invited Speaker, Gordon Summer Research Conference on “Microbial Toxins and Pathogenesis”. Alison O'Brien, Chair
1996 Invited Speaker, Regional Pediatric Gastroenterology Research Symposium. Joel Adelson, Chair. Brown University
1997 Invited Speaker, Keystone Symposia on “Mucosal Immunity: Cellular and molecular cross-talk at mucosal surfaces”, Marian Neutra and Martin Kagnoff, Chairs.
1997 Research Seminar, Dept. of Microbiology, Univ. of Colorado Health Sciences, Denver . R. Holmes, Chair.
1997 Visiting Professor: Cell Biology/Membrane Biology Seminar Series. Hospital for Sick Children, Univ. of Toronto . Hosts, Dept. of Cell Biology and Div. of Gastroenterology.
1997 Visiting Professor: Departments of Microbiology and Clinical Immunology. Microbiology Seminar Series. Mount Sinai Medical Center , NYC. Host: Dept. of Microbiology.
1997 Invited Speaker: Gastroenterology Research Group Symposium entitled Local Regulation of Epithelial Function: Transport, Immune Function, and Differentiation.
1997 Platform Presentation: 33rd US/Japan Joint Conference on Cholera and Related Diarrheal Diseases. NIH Sponsored Conference.
1998 Invited Speaker: Epithelial Polarity, American Society of Tropical Medicine and Hygiene.
1998 Invited Speaker and Visiting Professor: GI Cell Biology Symposium, Digestive Diseases Center at Washington University , St. Louis .
1999 Invited Speaker: Natural Pore Forming Proteins: Paneth Cell Cryptdins. American Chemical Society, Drug Delivery in the 21st Century
1999 Symposium Organizer and invited Speaker : Structure/Function of Caveoale in mammalian cells. Gastroenterology Research Group, American Gastroenterology Society Spring Meetings
2000 Invited Speaker: Keystone Conference on “Innate and Acquired Immunity at Mucosal Surfaces” Taos , NM
2000 Invited Speaker: Research Symposium on Microbial Pathogenesis. Gastroenterology Research Group, American Gastroenterology Society Spring Meetings
2000 Visiting Professor: Department of Microbiology, University of Michigan , Ann Arbor , MI
2001 Visiting Professor in Cell Biology, Univ. Virginia Sch. of Medicine
2002 Visiting Professor in Cell Biology, Univ. Texas South Western, UTSW
2002 Visiting Professor in Gastroenterology, Johns Hopkins Univ. Sch. of Medicine
2002 Visiting Professor: Department of Microbiology and Immunology University of Maryland School of Medicine
2002 Invited Seminar in "Bacterial Pathogenesis Series": Biochemistry Department, Holland Laboratories, Bethesda MD.
2002 Invited Faculty and Speaker, NASPGHAN Pediatric Gastroenterologists Research Forum, Scottsdale , Arizona
• Invited Speaker: Keystone Conference “Microbial, epithelial, lymphocyte interactions in mucosal immunity” Breckenridge , CO
2002 Invited Speaker: Seminar on Mucosal Vaccines, American Society of Microbiology National Spring Meetings, Salt Lake City , UT
2002 Visiting professor: Gastroenterology Section, University of Chicago .
2002 Visiting professor: “The Kango's Lectureship” Department of Pediatircs and the Department of Cell Biology, University of Pittsburg Medical School
2003 Visiting Professor: Gastroenterology, Univ. of Illinois
2003 Invited Symposium Speaker: Transport of Immunoglobulins across epithelial barriers, Spring Meetings of AGA
2003 Invited Symposium Speaker: Microbial Pathogenesis of cholera, Spring Meetings of AGA
2003 Invited Speaker: FASEB Summer Research Conference: GI Tract X: Physiology and pathophysiology of GI epithelia, Tucson AZ
2004 Invited Speaker: NIH Gastroenterology Center Symposium, University of Pennsylvania School of medicine
2004 Chair and Invited Speaker, Symposium on Entry of Bacterial Toxins into Host Cells, American Society of Microbiology, National Spring Meetings
2004 Visiting Professor: Department of Microbiology, Tufts University School of Medicine.
2004 Invited Speaker: Gordon Conference “Lysosomes”
2004 Visiting Professor: Emory University Medical School , Division of Gastroenterology and Nutrition
International
1993 Invited Speaker, Wellcome Centre for Medical Science: International Conference on "Cholera Toxin and Related Enterotoxins: From Disease to Molecular Structure". Dormy House, Worcestershire , England .
1999 Invited Speaker, ETOX9 International meetings on protein toxins. St. Maxime , France
1999 Invited Lecture, Institute Cochin Molecular Genetics, INSERM. Paris , France
• Invited Speaker: ETOX11 International Conference on the biology of microbial toxins, Prague , Cz
• Invited Speaker: ELSO European Cell Biology International Meetings, Nice, France
2004 Invited Speaker: The 34th Annual Meeting of the Japanese Society for Immunology, Sapporo , Japan
2005 Invited Speaker: INTERNATIONAL TITISEE CONFERENCE: Cell biology meets the immune system: molecular aspects of host pathogen interactions, Titisee, Germany Dr. Hidde Ploegh organizer.
REPORT of CLINICAL ACTIVITIES
Attending in Pediatric Gastroenterology and Nutrition and Nutrition Chief, Division of GI and Nutrition, Children's Hospital, Boston
PART III : BIBLIOGRAPHY
Original Papers
1. Lencer WI, Chu SH, Walker WA. 1987. Differential binding kinetics of cholera toxin to developing intestinal microvillus membrane. Infection and Immunity 55: 3126-3130.
2. Verkman A.S., Lencer W.I., Brown D., and D. Ausiello. 1988. Endosomes from kidney collecting duct contain the vasopressin-sensitive water channel. Nature 333: 268- 269.
3. Ye R., Shi L-b, Lencer W. I., and A. S. Verkman. 1989. Functional colocalization of water channels and proton pumps in endosomes from kidney proximal tubule. J. Gen. Physiol. 93: 885 - 902.
4. Lencer W. I., F. Reinhart, and M. R. Neutra. 1990. Interaction of cholera toxin with cloned human goblet cells in monolayer culture. Am. J. Physiol. (Gastrointest. Liver Physiol). 258: G96 - G102.
5. Lencer W. I., P. Weyer, A. S. Verkman, D. A. Ausiello, and D. Brown. 1990. FITC- dextran as a probe for endosome function and localization in kidney. Am. J. Physiol. (Cell Physiol.) 258: C309 - 317.
6. Shi L-B., R-G. Ye, W. I. Lencer, and A. S. Verkman. 1990. Characterization of proton pump and osmotic water transport in endocytic vesicles from rat kidney proximal tubule. Acta Physiologica Sinica 42: 105-113.
7. Lencer, W. I. , A. S. Verkman, A. Arnaout, D. Ausiello, and D. Brown. 1990. Endocytic Vesicles from Renal Papilla which Retrieve the Vasopressin-Sensitive Water Channel do not contain an H + ATPase. J. Cell Biol . 111: 379-389.
8. Lencer, W. I. , D. Brown, D. Ausiello, and A. S. Verkman. 1990. Endocytosis of water channels in rat kidney: cell specificity and correlation with in vivo antidiuresis. Am . J. Physiol . (Cell Physiol.) 259: C920-C932.
9. Madara, J. L., C. Parkos, S. Colgan, R. J. MacLeod, S. Nash, J. Matthews, C. Delp, and W. Lencer. 1992. Cl - secretion in a model intestinal epithelium induced by a neutrophil derived secretagogue. J. Clin. Invest . 89: 1938-1944.
10. Lencer, W. I. , C. Delp, M. R. Neutra, J. L. Madara. 1992. Mechanism of cholera toxin action on a polarized human intestinal cell line: role of vesicular transport. J. Cell Biol. 117: 1197-1209.
11. Kendall, D., Lencer, W., and K. Matlin. 1992. Cryosectioning of epithelial cells grown on permeable supports. J. Tissue Culture Methods 14: 181-186.
12. Lencer, W. I., J. B. de Almeida, S. Moe, J. L. Stow, D. A. Ausiello, and J. L. Madara. 1993. Entry of cholera toxin into polarized human intestinal epithelial cells: Identification of an early brefeldin A sensitive event required for A 1 -peptide generation. J. Clin. Invest. 92: 2941-2951.
13. Apter, F. M., W. I. Lencer, R. A. Finkelstein, J. J. Mekalanos and M. R. Neutra. 1993. Monoclonal antibodies directed against cholera toxin prevent the toxin-induced secretory response and block toxin binding to intestinal epithelial cells in vitro . Infect. Immun . 61: 5271-5278.
14. Dosanjh, A., W. I. Lencer, D. Brown, D. A. Ausiello, J. L. Stow. 1994. Heterologous expression of ?F508 CFTR results in decreased sialylation of membrane glycoconjugates. Am. J. Physiol (Cell) . 266: C360-C366.
15. Strohmeier, G. R., Reppert, S., Lencer, W. I. , and J. L. Madara. 1995. The A 2b adenosine receptor mediates cAMP responses to adenosine receptor agonists in human intestinal epithelia. J. Biol. Chem. 270: 2387-2394.
16. Lencer, W. I., G. Strohmeier, S. Moe, S. L. Carlson, C. T. Constable, and J. L. Madara. 1995. Signal transduction by cholera toxin in polarized epithelia: processing in vesicular compartments does not require acidification. Am. J. Physiol. 269: G548-G557.
17. Lencer, W. I. , S. Moe, P. A. Rufo, and J. L. Madara. 1995. Transcytosis of cholera toxin subunits across model human intestinal epithelia. Proc. Natl. Acad. Sci. USA. 92: 10094-10098.
18. Lencer, W. I., C. Constable, S. Moe, M. Jobling, H. M. Webb, S. Ruston, J. L. Madara, T. Hirst, and R. Holmes. 1995. Targeting of cholera toxin and E. coli heat labile toxin in polarized epithelia: role of C-terminal KDEL. J. Cell Biol. 131: 951-962.
19. Turner, J.R., W. I. Lencer, S. Carlson, and J. L. Madara. 1996. Carboxy terminus VSV-G tagged intestinal Na+-glucose cotransporter (SGLT1): Maintenance of surface expression and global transport function with selective perturbation of transport kinetics and polarized expression. J. Biol. Chem. 271: 7738-7744.
20. Frey, A., K. T. Giannasca, R. Weltzin, P. J. Giannasca, H. Reggio, W. I. Lencer, and M. R. Neutra. 1996. Role of the glycocalyx in regulating access of microparticles to apical plasma membranes on intestinal epithelial cells: implications for microbial attachment and oral vaccine targeting. J. Exp. Med . 184: 1045 - 1059.
21. Rufo, P. A., L. Jang, S. J. Moe, C. Brungara, S. L. Alper, and W. I. Lencer. 1996. The antifungal antibiotic clotrimazole inhibits Cl- secretion by polarized monolayers of human colonic epithelial cells. J. Clin. Invest. 98: 2066 - 2075.
22. Strohmeier, G. R., W. I. Lencer, T. W. Patapoff, L. F. Thompson, S. L. Carlson, S. J. Moe, D. Carnes, R. J. Mrsny, and J. L. Madara. 1997. Surface expression, polarization, and functional significance of CD73 in human intestinal epithelia. J. Clin. Invest . 99: 2588-2601.
23. Lencer, W. I., G. Cheung, G. R. Strohmeier, M. Currie, A. J. Ouellette, M. E. Selsted, and J. L. Madara. 1997. Induction of epithelial Cl secretion by channel forming cryptdins 2 and 3. Proc. Natl. Acad. Sci. USA . 94: 8585-8589. (Anonymous Submission)
24. Lencer, W. I., C. Constable, S. Moe, P. A. Rufo, A. Wolf, M. G. Jobling, S. P. Ruston, J. L. Madara, R. K. Holmes, and T. R. Hirst. 1997. Proteolytic activation of cholera toxin and Escherichia coli labile toxin by entry into host epithelial cells: signal transduction by a protease-resistant toxin variant. J. Biol. Chem . 272: 15562-15568.
25. Rufo, P. A., D. Merlin, M. Riegler, M. Ferguson-Maltzman, B. Dickinson, C. Brugnara, S. L. Alper, and W. I. Lencer. 1997. The antifungal antibiotic clotrimazole inhibits chloride secretion by human T84 cells via blockade of distinct basolateral K + conductances. J. Clin. Invest . 100: 3111-3120.
26. Wolf, A. A., M. G. Jobling, S. Wimer-Mackin, J. L. Madara, R. K. Holmes, and W. I. Lencer. 1998. Ganglioside structure dictates signal transduction by cholera toxin in polarized epithelia and association with caveolae-like membrane domains. J. Cell Biol . 141:917-927.
27. Rodighiero, C., A.T. Aman, M.J. Kenny, J. Moss, W.I. Lencer, and T.R. Hirst. 1999. Structural basis for the differential toxicity of cholera toxin and E. coli heat-labile enterotoxin. J. Biol. Chem . 274:3962-3969.
28. Beauregard, K., S. Wimer-Mackin, R.J. Collier, and W.I. Lencer. 1999. Entry of anthrax toxin into a polarized human epithelial cell line: strict dependence on basolateral membrane receptors. Infect. Immun . 67: 3026-3030.
29. Merlin, D., L. Jiang, G.R. Strohmeier, A. Nusrat, S.L. Alper, W.I. Lencer, and J.L. Madara. 1998. Distinct Ca 2+ - and cAMP-dependent anion conductances in the apical membrane of polarized T84 cells. Am. J. Physiol. (Cell Physiol. ) . 275:C484-C495.
30. Dickinson, B., K. Badizadegan, Z. Wu, J.C. Ahouse, X. Zhu, N.E. Simister, R.S. Blumberg, and W.I. Lencer. 1999. Bidirectional FcRn-dependent IgG transport in a polarized human intestinal cell line. J. Clin. Invest. 104: 903-911.
31. Badizadegan, K., B.L. Dickinson, H.E. Wheeler, R.S. Blumberg, R. K. Holmes, and W.I. Lencer. 2000. Hetergeneity of detergent insoluble membranes from human epithelia containing caveolin-1 and ganglioside G M1 . Am. J. Physiol . 278: G895-G904.
32. Mel, S.F., K.J. Fullner, S. Wimer-Mackin, W.I. Lencer, and J.J. Mekalanos. 2000. Association of Protease Activity in Vibrio cholerae Vaccine Strains with Decreases in Transcellular Epithelial Resistance of Polarized T84 Intestinal Epithelial Cells. Infect. Immun . 68: 6487-92.
33. Merlin, D., W.I. Lencer , M.E. Selsted, and J.L. Madara. 2000. Cryptdin-3 induces novel apical anion conductances in CT secretory epithelia. Am. J. Physiology (Cell ) . 280: C296-302
34. Tsai, B., C. Rodighiero, W.I. Lencer, and T. Rapoport. 2001. Protein disulfide isomerase acts as a redox-dependent chaperone to unfold cholera toxin. Cell . 104:937-948.
35. Wimer-Mackin, S., R.K. Holmes, A.A. Wolf, W.I. Lencer, and M.G. Jobling. 2001. Characterization of receptor-mediated signal transduction by Escherichia coli type IIa heat-labile enterotoxin in the polarized human intestinal cell line T84. Infect Immun. 69:7205-12.
36 Zhu, X., G. Meng, B.L. Dickinson, X. Li, E. Mizoguchi, L. Miao, Y. Wang, C. Robert, B. Wu, P.D. Smith, W.I. Lencer, and R.S. Blumberg. 2001. MHC class I-related neonatal Fc receptor for IgG is functionally expressed in monocytes, intestinal macrophages, and dendritic cells. J Immunol. 166:3266-76.
37. Merlin, D., W.I. Lencer , M.E. Selsted, and J.L. Madara. 2001. Cryptdin-3 induces novel apical anion conductances in CT secretory epithelia. Am. J. Physiology (Cell) . 280:C296-302.
38. Aman, A. T., S. Fraser, E.A. Merritt, C. Rodigherio, M.J. Kenny, M. Ahn, W.G. Hol, N.A. Williams, W.I. Lencer, and T.R. Hirst. 2001. A mutant cholera toxin B-subunit that binds GM1-ganglioside but lacks immunomodulatory or toxic activity. Proc Natl Acad Sci U S A. 98: 8536-41.
39. Fullner, K.J., W.I. Lencer, and J.J. Mekalanos. 2001. Vibrio cholerae-induced cellular responses of polarized T84 intestinal epithelial cells are dependent on production of cholera toxin and the RTX toxin. Infect Immun. 69:6310-7.
40. Rodighiero, C., Y. Fujinaga, T.R. Hirst, and W.I. Lencer. 2001. A cholera toxin B-subunit variant that binds ganglioside G M1 but fails to induce toxicity. J Biol Chem . 276:36939-45.
41. Wolf, A. A. Y. Fujinaga, and W I. Lencer. 2002. Uncoupling of the cholera toxin G M1 ganglioside-receptor complex from endocytosis, retrograde Golgi trafficking, and downstream signal transduction by depletion of membrane cholesterol. J. Biol. Chem . 277:16249-56.
42. Yue, G., D. Merlin, M.E. Selsted, W.I. Lencer, J.L. Madara, and D.C. Eaton. 2002. Cryptdin 3 forms anion selective channels in cytoplasmic membranes of human embryonic kidney cells. Am J Physiol Gastrointest Liver Physiol . 282:G757-65.
43. Claypool, S.M., Dickinson , B.L., Yoshida, M., Lencer, W.I., and Blumberg, R.S. 2002. Functional reconstitution of human FcRn in Madin-Darby canine kidney cells requires co-expressed human beta 2-microglobulin. J Biol Chem 277:28038-28050 .
44. Spiekermann, G.M., Finn, P.W., Ward, E.S., Dumont, J., Dickinson , B.L., Blumberg, R.S., and Lencer, W.I. 2002. Receptor-mediated immunoglobulin G transport across mucosal barriers in adult life: functional expression of FcRn in the mammalian lung. J Exp Me d 196:303-310.
45. Zhu, X., J. Peng, R. Raychowdhury, A. Nakajima, W.I. Lencer, and R.S. Blumberg. 2002. The heavy chain of neonatal Fc receptor for IgG is sequestered in endoplasmic reticulum by forming oligomers in the absence of beta2-microglobulin association. Biochem J. 367:703-14.
46. Rodighiero, C., Tsai, B., Rapoport, T. A., and Lencer, W. I. 2002. Role of ubiquitination in retro-translocation of cholera toxin and escape of cytosolic degradation. EMBO Reports 3, 1222-1227.
47. Shah, U., B.L. Dickinson, R.S. Blumberg, N.E. Simister, W.I. Lencer, and W.A. Walker. 2003. Distribution of the IgG Fc receptor, FcRn, in the human fetal intestine. Pediatr Res. 53:295-301.
48. Fujinaga, Y., A.A. Wolf, C. Rodigherio, H. Wheeler, B. Tsai, L. Allen, M.G. Jobling, T. Rapoport, R.K. Holmes, and W.I. Lencer. 2003. Gangliosides that associate with lipid rafts mediate transport of cholera toxin from the plasma membrane to the ER. Mol Biol Cell. 14: 4783-93
49. Tsai, B., J.M. Gilbert, T. Stehle, W. Lencer, T.L. Benjamin, and T.A. Rapoport. 2003. Gangliosides are receptors for murine polyoma virus and SV40. EMBO J. 22:4346-4355.
50. Rufo, P.A., P.W. Lin, A. Andrade, L. Jiang, L. Rameh, C. Flexner, S.L. Alper, and W.I. Lencer. 2004. Diarrhea-associated HIV-1 aspartyl protease-inhibitors potentiate muscarinic activation of Cl- secretion by T84 cells via prolongation of cytosolic Ca2+ signaling. Am. J. Physiol. Cell Physiol . 286: C998-C1008.
51. Claypool, S., B. Dickinson, J. Wagoner, W.I. Lencer*, and R.S. Blumberg*. 2004. Bidirectional transport of IgG by a strongly polarized basolateral Fc-receptor. Mol Biol Cell. 15: 1746-1759. (*Shared senior author)
52. Lin, P.W., Simon, P.O., Gewirtz, A.T., Neish, A.S., Ouellette, A.J., Madara, J.L. and Lencer, W.I. (2004) Paneth cell cryptdins act in vitro as apical paracrine regulators of the innate inflammatory response. J Biol Chem . 279: 19902-7.
53. Massol, R.H., J.E. Larsen, Y. Fujinaga, W.I. Lencer, and T. Kirchhausen. 2004. Cholera Toxin toxicity does not require functional Arf6- and dynamin-dependent endocytic pathways. Mol Biol Cell. 15:3631-41.
54. Feng, Y., A.P. Jadhav, C. Rodigherio, Y. Fujinaga, T. Kirchhausen, and W.I. Lencer. 2004. Retrograde transport of cholera toxin from the plasma membrane to the ER requires the TGN but not the Golgi apparatus in Exo2 treated cells. EMBO Rep . 5:596-601.
55. Bitonti, A.J., J.A. Dumont, S.C. Low, R.T. Peters, K. Kropp, V. Palombella, J.M. Stattel, Y. Lu, C.A. Tan, J.J. Song, A.M. Garcia, N.E. Simister, G.M. Spiekermann, W.I. Lencer*, and R.S. Blumberg*. 2004. Pulmonary delivery of a novel erythropoietin Fc-fusion protein in non-human primates using an immunoglobulin transport pathway. Proc. Natl. Acad, Sci , USA . 26: 9763-8. (*Shared senior author)
56. Yoshida, M., S.M. Claypool, J.S. Wagner, E. Mizoguchi, A. Mizoguchi, D.C. Roopenian, W.I. Lencer, and R.S. Blumberg. 2004. Human neonatal Fc receptor mediates transport of IgG into luminal secretions for delivery of antigens to mucosal dendritic cells. Immunity . 6: 769-83.
57. Badizadegan, K., H.E. Wheeler, Y. Fujinaga, and W.I. Lencer. 2004. Trafficking of cholera toxin-ganglioside GM1 complex into Golgi and induction of toxicity depend on actin cytoskeleton. Am J Physiol Cell Physiol. 287:C1453-62.
58. Gilbert, J., J. Dahl, C. Riney, J. You, C. Cui, R. Holmes, W. Lencer, and T. Benjamin. 2005. Ganglioside GD1a Restores Infectibility to Mouse Cells Lacking Functional Receptors for Polyomavirus. J Virol. 79:615-8.
Proceedings of Meetings
1. Lencer, W.I., D. Merlin, A.J. Ouellette , M.E. Selsted, and J.L. Madara. 1999. Natural pore forming proteins: Paneth cell cryptdins. In Drug delivery in the 21st century . Vol. in press. R.J. Mrsny and K. Park, editors. American Chemical Society, Washington , D.C.
2. Hirst, T. R., S. Eaglestone, W. I. Lencer, T. O. Nashar, H. M. Webb, J. Yu. 1994. Bacterial and host interactions during the biogenesis, toxicity and immunogenisity of Escherichia coli heat-labile enterotoxin. Biochemical Society Transactions . 22: 306 - 309.
3. Badizedagan, K., A.A. Wolf, C. Rodighiero, M. Jobling, T.R. Hirst, R.K. Holmes, and W.I. Lencer. 2000. Floating cholera toxin into epithelial cells: Functional association with caveolae-like detergent insoluble membrane microdomains. International Journal of Medical Microbiology, Virology, Parasitology and Infectious Disease s . 290:.
4. Lencer, W.I. 2004. Retrograde transport of cholera toxin into the ER of host cells. Int J Med Microbiol . 293:491-4.
Invited Reviews, Editorials, and Book Chapters
1. Lencer WI, Walker WA. 1988. Malabsorbtion syndrome. in Ambulatory Pediatric Care. R.A. Dershewitz ed., 472-476.
2. Lencer WI. 1993. Malabsorbtion syndrome and Chronic Diarrhea. in Ambulatory Pediatric Care 2nd edition. R.A. Dershewitz ed., 411-415.
3. Lencer, W. I., J-F Desjeux. 1996. Transport of Water and Ions, in Pediatric Gastrointestinal Disease. 2nd Edition . W. A. Walker, P. Durie, R. Hamilton, J. Walker-Smith, and J. Watkins, editors.
4. Wolf, A. and W. I. Lencer WI. 1998. Malabsorbtion syndrome and Chronic Diarrhea. in Ambulatory Pediatric Care 3nd edition. R.A. Dershewitz ed.
5. Hirst, T. R., L. W. Ruddock, J. Hillary, and W. I. Lencer. 1998. Membrane translocation and targeting of cholera toxin and related enterotoxins in prokaryotic and eukaryotic systems. In Bacterial Protein Toxins . J. Hacker, editor. Gustav Fischer Verlag, Stuttgart .
6. Lencer, W. I. 1998. Acid secretion, ulcer disease, and cell biology. Gastroenterology 114: 607-608.
7. Lencer, W. I. 1998. Paneth cells: on the front line or in the backfield. Gastroenterology. 114:1343-1345.
8. Lencer, W. I. 1998. Guanylyl cyclase-G: an "orphan receptor" for signal transduction in the intestine. Gastroenterology . 115: 791-792.
9. Lencer, W. I. , and S. A. Alper. 1999. The potassium channel and how it works. Gastroenterology . 116: 216-217.
10. Lencer, W. I. 1999. Mucosal Vaccination: “All Politics are Local” U.S. Rep. Tip O'Neil. Gastroenterology . 116: 497-499.
11. Lencer, W.I. 1999. Floating the machinery for T cell activation. Gastroenterology. 117: 503-505.
12. Lencer, W.I., T.R. Hirst, and R.K. Holmes. 1999. Membrane traffic and the cellular uptake of cholera toxin. Biochim. Biophys. Acta. (Molecular Cell Research) . 1450: 177-190. (invited review)
13. Blumberg, R.S., W.I. Lencer, X. Zhu, H.-S. Kim, S. Claypool, S.P. Balk, L.J. Saubermann, and S.P. Colgan. 1999. Antigen presentation by intestinal epithelial cells. Immunology Letters. 69:7-11.
14. Lencer, W.I. 1999. Signaling to colon cancer. Gastroenterology . 117:1499-1505.
15. Lencer, W.I., and M.R. Neutra. 2000. Salmonella pathogenesis: the Trojan horse or the New York shuttle? Gastroenterolog y . 118: 803-805.
16 Duggan, C., and W.I. Lencer. 2000. A novel development in oral rehydration therapy. Gastroenterolog y . 119: 591-592.
17. Lencer, W.I. 2000. Microbial invasion across the epithelial barrier. Pediatric Research . 49:4-5.
18. Lencer, W.I. 2001. Cholera: Invasion of the intestinal epithelial barrier by a stably folded protein toxin. Am J Physiol Gastrointest Liver Physiol. 280:G781-6. (invited review)
19.. Blumberg, R.S., Y. van de Wal, S. Claypool, N. Corazza, B. Dickinson, E. Nieuwenhuis, R. Pitman, G. Spiekermann, X. Zhu, S. Colgan, and W.I. Lencer. 2001. The multiple roles of major histocompatibility complex class-I-like molecules in mucosal immune function. Acta Odontol Scand. 59:139-44.
20. Badizadegan, K., Collier, R.J., and Lencer, W.I. 2002. Membrane translocation by bacterial toxins. In Methods in Microbiology . P. Sansonetti, and A. Zychlinsky, editors. San Diego : Academic Press. 277-296.
21. Bonny L. Dickinson and Wayne I. Lencer. 2003. Transcytosis of Bacterial Toxins Across Mucosal Barriers in Bacterial Protein Toxins D, Burns, J. Barbieri, B. H. Iglewski, and R. Rappuoli editors. ASM Press. Washington D.C. .
22. Chiara Rodighiero and Wayne I. Lencer. 2003. "Trafficking of cholera and related bacterial enterotoxins: pathways and endpoints" in Microbial Pathogens and the Intestinal Epithelial Cell . Gail Hecht, editor, ASM Press.
23. Lencer, W.I., K. Barrett, S. Colgan, M.B. Cohen, C.P. Duggan, M. Kotelchuck, and J.M. Rhoads. 2002. Research Agenda for Pediatric Gastroenterology, Hepatology and Nutrition: Secretion and Diarrhea: Report of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition for the Children's Digestive Health and Nutrition Foundation. J Pediatr Gastroenterol Nut r. 35 Suppl:S246-9.
24. Lencer, W.I., and B. Tsai. 2003. The intracellular voyage of cholera toxin: Going retro. Trends Biol Chem . 28: 639-645. (invited review)
25. Lencer, W.I., and R.S. Blumberg. 2004. A passionate kiss and-then-run: exocytosis and recycling of IgG by FcRn. Trends Cell Biol. in press. (invited commentary)
26. Saslowsky, D.E., M. Kothe, and W.I. Lencer. 2004. Cholera toxin: mechanisms of entry into host cells. Topics in Current Genetics. in press. (invited review)
Education Materials
1. HST120 Course syllabus GI Pathophysiology (revised each year since 1995)
2. Integrated Human Physiology (HMS year 1) (revised each year since 1998)
Patents
PAT. NO. Title
1. US 6,495,567 Triaryl methane compounds and their use in the treatment of diarrhea and scours
2. US 6,485,726 Receptor specific transepithelial transport of therapeutics
3. US 6,291,449 Use of 11-phenyl-dibenzazepine compounds to treat diarrhea or scours
4. US 6,086,875 Receptor specific transepithelial transport of immunogens
5. US 6,030,613 Receptor specific transepithelial transport of therapeutics
6. US 5,889,038 Methods and products for treating diarrhea and scours: use of clotrimazole and related aromatic compounds
7. EU 0910376 Use of clotrimazole and related compounds in the treatment of diarrhea
8. DE 696 27 820 T2 Receptor specific transepithelial transport of immunogens
